155-050

Cellular ATP

4294 SEK

For analyzing  mammalian cell samples as HTS

Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.

In samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.

 

144-041

ATP Kit SL

3470 SEK

For assays of both enzymes and metabolites

ATP Kit SL is intended for assays of enzymes and metabolites by monitoring the formation or degradation of ATP (adenosine triphosphate) over the range of 10 –12 to 10 –6 mol/L.

Our technology

BioThema is entirely focused on analytical applications of the firefly luciferase reaction whether it is detecting cells, measuring enzyme activity or something else. In this reaction luciferin is activated with ATP (adenosine triphosphate) and thereafter oxidized to oxyluciferin and CO2. The oxyluciferin is formed in an excited state and emits light when going to its…