D-Luciferin is used for in-vivo and in-vitro studies of firefly luciferase and high quality luciferin is a vital component of BioThemas kits. BioThema offers D-luciferin free acid, D-Luciferin Potassium salt (D-Luciferin K-salt) and D-Luciferin Sodium salt (D-Luciferin Na-salt) and L-luciferin.
DetailsLuminescent substrate for firefly luciferase
D-Luciferin is used for in-vivo and in-vitro studies of firefly luciferase and high quality luciferin is a vital component of BioThemas kits. BioThema offers D-luciferin free acid, D-Luciferin Potassium salt (D-Luciferin K-salt) and D-Luciferin Sodium salt (D-Luciferin Na-salt) and L-luciferin.
DetailsLuminescent substrate for firefly luciferase
D-Luciferin is used for in-vivo and in-vitro studies of firefly luciferase and high quality luciferin is a vital component of BioThemas kits. BioThema offers D-luciferin free acid, D-Luciferin Potassium salt (D-Luciferin K-salt) and D-Luciferin Sodium salt (D-Luciferin Na-salt) and L-luciferin.
Details
Lysing Diluent is used to release ATP from mammalian cells. It contains an ATPase inhibitor, to avoid degradation of the extracellular ATP after the cells have been opened.
Cell Lysing Reagent is intended for the extraction of ATP from mammalian cells. It contains a milder detergent than Extractant B/S and will not affect bacterial cells. If Cell Lysing Reagent is reconstituted in 71-101 ATP Eliminating Reagent extracellular ATP will be eliminated as well, for cases when only bacterial ATP is of interest.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
Cell Viability Kit SL is intended for use with mammalian cell samples with high cell density or with cell samples with higher density of ATPases, e.g. nerve cells. The kit contains a stronger ATPase inhibitor than 155-050 Cellular ATP Kit HTS and produces stable light.
A mammalian cell contains approx. 1 femtomole ATP. 188-441 Cell Viablity Kit SL is useful for quantification of ATP in the range 10^-11– 10^-7 mole/L. This kit can be used for determination of ATP levels in e.g. animal or bacterial cells in a variety of circumstances:
Estimation of cell numbers
Cell proliferation studies
Cytotoxicity studies