Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.
In samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.
10 mL, Reconstitution buffer for ATP Reagent HS used with Extractant B/S
Diluent B is a reconstitution buffer for 12-101 ATP Reagent HS when Extractant B/S has been used. Diluent B neutralizes the enzyme inhibiting component in the extractant, allowing full activity of luciferase.
6.7 mL, Reconstitution buffer for ATP Reagent HS used with Extractant B/S
Diluent B (dropper bottle) is a reconstitution buffer for 12-071 ATP Reagent HS. The reagent is intended for hygiene control and the diluent is therefore delivered in a practical dropper bottle. The dropper bottle allows personel without any training in pipetting technique to perform the assay rapidly and easily.
10 mL, Reconstitution buffer for 12-101 ATP Reagent HS, when Extractant B/S has not been used.
Diluent D is a reconstitution buffer for 12-101 ATP Reagent HS, when Extractant B/S has not been used. If Extractant B/S has been used, please choose 22-101 Diluent B instead, to avoid deactivation of the luciferase.
10 mL reagent for extraction of ATP from bacterial and mammalian cells.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
100 mL reagent for extraction of ATP from bacterial and mammalian cells.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
