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Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
Extractant B/S is used for the extraction of ATP from bacterial and mammalian cells. It is a strong extractant and is suitable for use with all bacterial cells and most types of other cells. It rapidly neutralizes all ATPases. To avoid degradation of luciferases the ATP Reagent should be reconstituted in Diluent B to neutralize the extractant.
The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances provided it is not important to degrade ATP in somatic cells:
1. Bacteriological control of liquids (e. g. drinking water, process water, beverages and beer)
2. Pharmaceuticals and cosmetics
3. Cell adhesion to surfaces
4. Quality assurance of laundry
5. Bacterial growth.
6. Antibiotic effects on bacteria
Kinase RR Kit or Kinase Reaction rate Kit is developed for the easy and reliable identification of protein kinase inhibitor candidates from large compund libraries. The Kinase RR Kit uses direct bioluminescent measurement of ATP degradation rate to distingusih hits from non-hits. In contrast to other bioluminescent ATP based assays of kinase there is no interference from inhibitors acting on luciferase. Furthermore the standard curve is linerar rather than sigmodial. the Kinase RR Kit is flexible and can easily be adapted to various HTS strategies. Z’ values up to 0.96 have been reported. The assay does not require radioactive materials, antibodies or any form of conjugates.
L-luciferin is a potent competitive inhibitor of firefly luciferase.
Luciferase assay HTS Kit w/o disp. is suitable when a luminometer without dispensers is available and you want to perform an HTS assay.
Luciferase assay HTS Kit w/ disp. is suitable when a luminometer with dispensers is available and you want to perform an HTS assay.
484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:
1) Firefly luciferase is not present in normal cells.
2) The assay is simple and easy to perform.
3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.
4) It is suitable for HTS.
The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.
The reagent contains D-Luciferin and Luciferase activators to obtain a stable light (less than 2% decay per minute).
The reagent contains D-Luciferin and Luciferase activators to obtain a stable light (less than 2% decay per minute).
