Luciferase assay Kit

484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:

1) Firefly luciferase is not present in normal cells.

2) The assay is simple and easy to perform.

3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.

4) It is suitable for HTS.

The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.

The firefly luciferase luc gene is the most commonly used reporter gene for studying cellular events coupled to gene expression. It has a number of distinct advantages such as: no background, easy analytical procedure, extreme sensitivity (10^-20 mole luciferase can be detected) and reliability of results. The reagents contain special activators to counteract any inactivation of the luciferase enzyme during cell culture or sample storage. All three Luciferase Assay Kits contain the same reagents. The High Throughput Screening kits (484-102 and 484-103) come in larger vials for easier handling.

The stable light makes it possible to use manual single tube luminometers as well as microplate luminometers. The detection limit obviously depends on the luminometer. With most luminometers 10 ^-19 moles of luciferase can be detected.

Detailed described procedures are found in the “Instructions for Use”, which is included with every kit.

Detection limit:

With most luminometers 10 ^-19 moles of luciferase can be detected.