Antibodies or antigens may be labelled with luciferase or ATP producing enzymes. Biotinylated recombinant luciferase can be detected at 10^-20 mol and biotinylated acetate kinase at 10-^21 mol.
- Sensitive detection of antibodies
- Sensitive detection of antigens
Luciferase Assay Kit, Luciferase Assay Kit HTS, ATP Kit SL
Murakami, S, Nakajima, M, Sekine, H, Maeda, M, Tsuji, A (1996) Streptavidin-biotin based bioluminescent enzyme immunoassay using biotinylated acetate kinase and recombinant firefly luciferase. Analytical Letters 29, 969-980.
Murakami, S., Ito, K., Goto, T., Kamada, S., Maeda, M. (1998) Bioluminescent enzyme immunoassay using thermostable mutant luciferase and acetate kinase as a labelled enzyme. Anal. Chim. Acta 361, 19-26
Fukuda, S, Tatsumi, H, Maeda, M (1998) Bioluminescent enzyme immunoassay with biotinylated firefly luciferase. Journal of Clinical Ligand Assay 21, 358-362.