Showing 1–10 of 12 results

266-311

ATP Biomass Kit HS

3365kr

For determination of the total ( both extracellular and intracellular) amount of ATP in a sample.

The ATP Biomass Kit is suitable for virtually all types of samples.

The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances:
1 Liquids (e. g. drinking water, process water, environmental samples, beverages and beer)
2 Pharmaceuticals and cosmetics
3 Cell adhesion to surfaces (e. g. biocompatibility studies)
4 Quality assurance of laundry
5 Bacterial growth.
6 Antibiotic effects on bacteria

266-221

ATP Hygiene Kit HS

2039kr

For hygiene control of surfaces and liquids

The  ATP Hygiene Kit HS is suitable for hygiene control of surfaces and liquids (e.g. rinsing water). Practical dropper bottles makes the kit easy-to-use, even for personel not used to handling pipettes.

ATP measurement is an excellent way of controlling hygiene, as ATP is found in food residues and other biological dirt as well as living cells. Even though a surface might be sterile (sterile=no bacteria are present) there might still be food residues left after cleaning. Such residues maks an excellent environment for airborne bacteria to grow and in a few hours the surface will no longer be sterile. With the ATP Hygiene Kit HS you can determine not only how many living organisms are present, but also the amount of biological dirt.

144-041

ATP Kit SL

3207kr

For assays of both enzymes and metabolites

ATP Kit SL is intended for assays of enzymes and metabolites by monitoring the formation or degradation of ATP (adenosine triphosphate) over the range of 10 –12 to 10 –6 mol/L.

188-441

Cell viability Kit SL

6943kr

For analyzing cell samples with high cell density

Cell Viability Kit SL is intended for use with mammalian cell samples with high cell density or with cell samples with higher density of ATPases, e.g. nerve cells. The kit contains a stronger ATPase inhibitor than 155-050 Cellular ATP Kit HTS and produces stable light.

A mammalian cell contains approx. 1 femtomole ATP. 188-441 Cell Viablity Kit SL is useful for quantification of ATP in the range 10^-11– 10^-7 mole/L. This kit can be used for determination of ATP levels in e.g. animal or bacterial cells in a variety of circumstances:
Estimation of cell numbers
Cell proliferation studies
Cytotoxicity studies

155-050

Cellular ATP

3968kr

For analyzing  mammalian cell samples as HTS

Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.

In samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.

 

266-111

Intracellular ATP Kit HS

4125kr

Determination of intracellular ATP in bacterial cells

The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances provided it is not important to degrade ATP in somatic cells:
1. Bacteriological control of liquids (e. g. drinking water, process water, beverages and beer)
2. Pharmaceuticals and cosmetics
3. Cell adhesion to surfaces
4. Quality assurance of laundry
5. Bacterial growth.
6. Antibiotic effects on bacteria

111-021

Kinase RR Kit

2596kr

Identification of protein kinase inhibitor

Kinase RR Kit or Kinase Reaction rate Kit is developed for the easy and reliable identification of protein kinase inhibitor candidates from large compund libraries. The Kinase RR Kit uses direct bioluminescent measurement of ATP degradation rate to distingusih hits from non-hits. In contrast to other bioluminescent ATP based assays of kinase there is no interference from inhibitors acting on luciferase. Furthermore the standard curve is linerar rather than sigmodial. the Kinase RR Kit is flexible and can easily be adapted to various HTS strategies. Z’ values up to 0.96 have been reported. The assay does not require radioactive materials, antibodies or any form of conjugates.

484-102

Luciferase assay HTS Kit w/o disp.

18364kr

For HTS reporter gene assay without a dispenser equipped luminometer, 4800 assays

Luciferase assay HTS Kit w/o disp. is suitable when a luminometer without dispensers is available and you want to perform an HTS assay.

484-103

Luciferase assay HTS w/ disp.

18364kr

For HTS reporter gene assay with a dispenser equipped luminometer, 4600 assays

Luciferase assay HTS Kit w/ disp. is suitable when a luminometer with dispensers is available and you want to perform an HTS assay.

484-001

Luciferase assay Kit

3662kr

For reporter gene assay allowing the monitoring of promoter activity in the control of gene expression.

484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:

1) Firefly luciferase is not present in normal cells.

2) The assay is simple and easy to perform.

3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.

4) It is suitable for HTS.

The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.

BioThema has kits for all kinds of different applications. Choose your application area below or have a look at our Kit selection guide.