Methodology:
The luc gene coding for firefly luciferase may be attached to another gene of interest. The expressing of this gene may then be estimated from the level of luciferase expression measured as light. This may be done in vitro, i.e. after lysing the cells adding ATP and D-luciferin, or in vivo. In the latter case D-luciferin has to enter the cells. Even whole live animals or plants may be studied, “in vivo imaging”, by injecting D-luciferin into the organism (ATP is already in the cells).

Intended use:

  1. In an easy and sensitive way measure the expression of a gene of interest under various conditions by measuring the expression of luciferase.
  2. Follow how luminescent cells or viruses spread in e.g. an animal.

Suitable products for in vitro measurements: Luciferase Assay Kit, Luciferase Assay Kit HTS

Suitable products for in vivo measurements: Endotoxin free D-luciferin, potassium salt

Recommended papers:

Lundin, A. (1993) Optimised assay of firefly luciferase with stable light emission. In: Bioluminescence and Chemiluminescence (Szalay, A., Kricka, L. J. and Stanley, P., Eds.), pp. 291-295, John Wiley & Sons, Chichester.

Lundin A. (2014) Optimization of the firefly luciferase reaction for analytical purposes. Adv. Biochem. Engin./Biotechnol. 2014; 145:31-62

 

El-Sayed, R., Eita, M., Barrefelt, Å., Ye, F., Jain, H., Fares, M., Lundin, A., Crona, M., Abu-Salah, K., Muhammed, M., and Hassan, M. Thermostable Luciferase from Luciola cruciate for Imaging of Carbon Nanotubes and Carbon Nanotubes Carrying Doxorubicin Using in Vivo Imaging System. (2013) Nano Lett. 13, 1393−1398

Please choose the kit best describing your assay.

484-001

Luciferase assay Kit

3662kr

For reporter gene assay allowing the monitoring of promoter activity in the control of gene expression.

484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:

1) Firefly luciferase is not present in normal cells.

2) The assay is simple and easy to perform.

3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.

4) It is suitable for HTS.

The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.

484-103

Luciferase assay HTS w/ disp.

18364kr

For HTS reporter gene assay with a dispenser equipped luminometer, 4600 assays

Luciferase assay HTS Kit w/ disp. is suitable when a luminometer with dispensers is available and you want to perform an HTS assay.

484-102

Luciferase assay HTS Kit w/o disp.

18364kr

For HTS reporter gene assay without a dispenser equipped luminometer, 4800 assays

Luciferase assay HTS Kit w/o disp. is suitable when a luminometer without dispensers is available and you want to perform an HTS assay.