Antibiotic susceptibility as well as antibiotic concentration can be determined using luciferase based assays. Tests are rapid (1.5-2 hours) and measure inhibition of growth or release of extracellular ATP. With slow-growers like mycobacteria susceptibility testing takes 7 days.

Products: 266-311 ATP Biomass Kit HS and 266-112 Microbial ATP Kit HS

Publications: L E Nilsson, S E Hoffner and S Ånséhn Rapid Susceptibility Testing of Mycobacterium tuberculosis by Bioluminescence Assay of Mycobacterial ATP; ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Aug. 1988, p. 1208-1212)

Please choose the kit best describing your assay.

144-041

ATP Kit SL

3470 SEK

For assays of both enzymes and metabolites

ATP Kit SL is intended for assays of enzymes and metabolites by monitoring the formation or degradation of ATP (adenosine triphosphate) over the range of 10 –12 to 10 –6 mol/L.

484-001

Luciferase assay Kit

3962 SEK

For reporter gene assay allowing the monitoring of promoter activity in the control of gene expression.

484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:

1) Firefly luciferase is not present in normal cells.

2) The assay is simple and easy to perform.

3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.

4) It is suitable for HTS.

The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.